Evaluation of the green synthesis, characterization and antibacterial activity of silver nanoparticles from corm extract of Crocus sativus var. Haussknechtii | ||
| Journal of Horticulture and Postharvest Research | ||
| مقاله 4، دوره 4، Special Issue - Recent Advances in Saffron - شماره پیاپی 13، پاییز 2021، صفحه 19-32 اصل مقاله (1.96 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22077/jhpr.2021.3812.1177 | ||
| نویسندگان | ||
| Navid Zivyar1؛ Ghodsieh Bagherzade* 1؛ Maryam Moudi2؛ Maryam Manzari tavakoli3 | ||
| 1Department of Chemistry, Faculty of Science, University of Birjand, Birjand 97179-414, Iran | ||
| 2Department of Biology, Faculty of Science, University of Birjand, Birjand 97179-414, Iran | ||
| 3Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran | ||
| چکیده | ||
| Purpose: To prevent chemical toxicity, biosynthesis of silver nanoparticles has been proposed as a cost-effective and environmentally friendly reducing agent option by corm extract of Crocus sativus var. Haussknechtii and as well as, evaluate their effects against Staphylococcus aureus and Escherichia coli. Research method: Silver nanoparticles were produced in the presence of secondary metabolites of this plant. The nanoparticles were then identified using the technique ultraviolet-visible spectroscopy (UV–Vis), fourier transform infrared (FTIR), field emission scanning electron microscope (FESEM).The antibacterial properties were used against two microorganisms, S. aureus (Gram-positive) and E. coli (Gram-negative), using the agar well propagation method. Findings: The observation of the peak at 450 nm in the UV-Vis spectra for corm synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. The optimum pH and AgNO3 concentrations were 9 and 4 mM, respectively. FESEM images detected the spherical Ag-NPs shape with diverse sizes ranged within 70.70–192.02 nm. Additionally, based on the antibacterial test that has been done for nanoparticles, the mean diameter of the inhibition zone after exposure to S. aureus and E. coli was 22.67±0.58 and 20.00±00 mm, respectively. Limitations: There was no significant limitation to report. Originality/Value: The corm extract of C. haussknechtii is a promising agent for the biosynthesis of almost spherical silver nanoparticles. The synthesized nanoparticles show good Inhibition activity in different concentrations. The AgNPs synthesized by corm extract in high concentrations are found to be high antibacterial activity against two bacterial organisms. This indicates that the increase in nanoparticle diameter is directly related to antimicrobial properties. | ||
| کلیدواژهها | ||
| Agar well method؛ Antibacterial activities؛ Crocus haussknechtii extract؛ Inhibiting activity؛ Silver nanoparticles | ||
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