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اثر نانو ذرات نقره بر صفات فیزیولوژیک برخی اکوتیپهای زعفران (.Crocus sativus L) خراسان جنوبی تحت تنش کمآبی ملایم | ||
تنشهای محیطی در علوم زراعی | ||
مقاله 14، دوره 11، شماره 3، مهر 1397، صفحه 627-643 اصل مقاله (3.4 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22077/escs.2017.701.1135 | ||
نویسندگان | ||
بتول صابرتنها* 1؛ براتعلی فاخری2؛ نفیسه مهدی نژاد3؛ زهره علیزاده4 | ||
1دانشآموخته کارشناسی ارشد اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
2دانشیار اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
3استادیار اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل | ||
4استادیار اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بیرجند | ||
چکیده | ||
این پژوهش بهمنظور بررسی تأثیر تنش کمآبی و نانو ذرات نقره بر صفات فیزیولوژی در گیاه زعفران انجام شد. آزمایش در دو سطح کمآبی و نرمال بر روی 10 اکوتیپ زعفران در سه سطح شاهد (آب مقطر)، 55و 110 پی پی ام نانو ذرات نقره پیاده شد. این بررسی بهصورت اسپلیت پلات فاکتوریل در قالب طرح بلوک های کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشگاه بیرجند و پژوهشکده زیستفناوری دانشگاه زابل انجام شد. بعد از تهیه نمونه های برگی از تمامی تیمارها، عصاره آنزیمی برای اندازه گیری میزان آنزیمها، کلروفیل a و b، کارتنوئید و پروتئین تهیه شد. داده ها با نسخه 9.2 نرمافزارSAS مورد تجزیهوتحلیل قرار گرفتند. اثرات اصلی تیمارهای اکوتیپ، نانوذره نقره، تنش خشکی و اثرات متقابل آنها برای آنزیمهای کاتالاز، پلی فنل اکسیداز، فنیل آلانین آمونیالیاز، کلروفیل b وکارتنوئید در سطح احتمال یک درصد معنی دار شدند. بیشترین مقدار کلروفیل b، کارتنوئید و فنیل آلانین آمونیالیاز تحت تنش خشکی در غلظت 55 پیپیام نانوذره نقره نسبت به شاهد و سطح 110 پی پی ام مشاهده شد که میتوان استدلال کرد نانو ذرات به دلیل خاصیت آبدوستی و آنتی باکتریال توانسته عملکرد بالایی داشته باشد. بیشترین مقدار کلروفیل a، کاتالاز، پلی فنل اکسیداز و گایاکول پراکسیداز تحت تنش خشکی در تیمار آب مقطر (شاهد) مشاهده شد که میتوان استدلال کرد نانو نقره در این صفات سمیت ایجاد کرده و باعث کاهش عملکرد در سطوح نانو نقره نسبت به شاهد شده است. درنتیجه اکوتیپهای قاین و آرین شهر در شرایط تنش خشکی تحت نانو ذرات نقره با غلظت 55 پی پی ام بیشترین عملکرد داشتند. | ||
کلیدواژهها | ||
الیسیتور؛ کاتالاز؛ کارتنوئید؛ کلروفیل | ||
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