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Optimization of polyphenol and flavonoids extraction from Ocimum gratissimum L. using response surface methodology | ||
| Journal of Horticulture and Postharvest Research | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 27 بهمن 1404 اصل مقاله (1.45 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22077/jhpr.2025.9175.1491 | ||
| نویسندگان | ||
| Tran Thien Hien* 1؛ Tran Trong Kieu Ngoc2؛ Nguyen Manh Ngoc Bao1؛ Vo Trung Tinh1؛ Pham Nguyen Thuy Dung1 | ||
| 1Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, 70000 Vietnam | ||
| 2Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, 70000 Vietnam | ||
| چکیده | ||
| Purpose: The study was conducted to optimize the extraction process with high polyphenol and flavonoid content from O. gratissimum L. and evaluate their biological activities. Research Method: The extraction of O. gratissimum L. extract was performed by combining ultrasound-assisted and hot extraction methods. Findings: The results showed that extraction of O. gratissimum L. was performed under optimal conditions by response surface methodology (RSM), including ethanol concentration of 70%, raw material: ethanol ratio of 1: 35.71 (g.g-1), time extraction of 69.77 min, and at a temperature of 89.82 °C, with a total polyphenol content and a total flavonoid content of 190.987 (mgGAE.gDW-1) and 27.10 (mgQE.gDW-1), respectively. The extract was concentrated by vacuum rotary evaporation to obtain the total extract which was subjected to evaluation for its physicochemical components and biological activities. The results showed that the total extract quality met the current standards of Vietnam without heavy metals and pesticide residues. In addition, the antibacterial, antioxidant and anti-inflammatory properties of the total extract sample from O. gratissimum L. also gave positive results, confirming the significant biological activity. Research limitations: No limitations were encountered. Originality/Value: Ocimum gratissimum L. is a medicinal herb widely used in traditional medicine across East Asia, especially in Vietnam. Due to the high content of physiochemical components, including essential oils and phenolic compounds such as polyphenols and flavonoids, O. gratissimum L. exhibits antioxidant, anti-inflammatory, and antibacterial properties. Therefore, studies on the biological activity of solution extracts and total extracts from O. gratissimum L. are essential for evaluating the potential applications in the fields of medicine, foods, and cosmetics. | ||
| کلیدواژهها | ||
| Antibacterial؛ Flavonoid؛ Ocimum gratissimum l؛ Polyphenol؛ RSM | ||
| مراجع | ||
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Adekola, M. B., Areola, J. O., Fagbohun, O. F., Asaolu, F. T., Ogundepo, G. E., Fajobi, A. O., & Babalola, O. O. (2022). In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig. Journal of Pharmaceutical Analysis, 12(2), 350–354. https://doi.org/10.1016/j.jpha.2021.04.002
Adelakun, S. A., Ogunlade, B., Akintunde, O. W., & Omilachi, V. O. (2022). Long-term exposure to cimetidine induced gonado-toxicity in male rats: modulating role of Ocimum gratissimum. Revista Internacional de Andrología, 20, S2–S16. https://doi.org/10.1016/j.androl.2020.10.012
Airaodion, A. I., Ibrahim, A. H., Ogbuagu, U., Ogbuagu, E. O., Awosanya, O. O., Akinmolayan, J. D., Njoku, O. C., Obajimi, O. O., Adeniji, A. R., & Adekale, O. A. (2019). Evaluation of phytochemical content and antioxidant potential of Ocimum gratissimum and Telfairia occidentalis leaves. Asian Journal of Research in Medical and Pharmaceutical Sciences, 1–11. https://doi.org/10.9734/ajrimps/2019/v7i130110
Ajayi, A., Ben-Azu, B., Onasanwo, S., Adeoluwa, O., Eduviere, A., & Ademowo, O. (2019). Flavonoid-Rich fraction of Ocimum gratissimum attenuates lipopolysaccharide-induced sickness behavior, inflammatory and oxidative stress in mice. Drug Research, 69(03), 151–158. https://doi.org/10.1055/a-0654-5042
Alabi, A. O., Ajayi, A. M., Omorogbe, O., & Umukoro, S. (2019). Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava. Clinical Phytoscience, 5(1), 34. https://doi.org/10.1186/s40816-019-0130-2
Alara, O. R., Abdurahman, N. H., Ukaegbu, C. I., & Alara, J. A. (2020). Optimization of microwave-assisted extraction of phenolic compounds from Ocimum gratissimum leaves and its LC–ESI–MS/MS profiling, antioxidant and antimicrobial activities. Journal of Food Measurement and Characterization, 14(6), 3590–3604. https://doi.org/10.1007/s11694-020-00602-1
Ali Redha, A. (2021). Review on extraction of phenolic compounds from natural sources using green deep eutectic solvents. Journal of Agricultural and Food Chemistry, 69(3), 878–912. https://doi.org/10.1021/acs.jafc.0c06641
Antony, A., & Farid, M. (2022). Effect of temperatures on polyphenols during extraction. Applied Sciences, 12(4), 2107. https://doi.org/10.3390/app12042107
Azizah, N. S., Irawan, B., Kusmoro, J., Safriansyah, W., Farabi, K., Oktavia, D., Doni, F., & Miranti, M. (2023). Sweet basil (Ocimum basilicum L.)―a review of its botany, phytochemistry, pharmacological activities, and biotechnological development. Plants, 12(24), 4148. https://doi.org/10.3390/plants12244148
Belwal, T., Dhyani, P., Bhatt, I. D., Rawal, R. S., & Pande, V. (2016). Optimization extraction conditions for improving phenolic content and antioxidant activity in Berberis asiatica fruits using response surface methodology (RSM). Food Chemistry, 207, 115–124. https://doi.org/10.1016/j.foodchem.2016.03.081
Bhavani, T., Mohan, R. R., Mounica, C., Nyamisha, J., Krishna, A. G., Prabhavathi, P., Raja, D. R. R., & Baba, D. K. H. (2019). Phytochemical screening & antimicrobial activity of Ocimum gratissimum review. Journal of Pharmacognosy and Phytochemistry, 8(2), 77–79.
Chopra, B., & Dhingra, A. K. (2021). Natural products: A lead for drug discovery and development. Phytotherapy Research, 35(9), 4660–4702. https://doi.org/10.1002/ptr.7099
Dharsana, J. N., & Mathew, S. M. (2014). Preliminary sceening of Anti inflammatory and Antioxidant activity of Morinda umbellata. International Journal of Pharmacy & Life Sciences, 5(8), 3774–3779.
Djuraev, K., Yodgorova, M., Usmonov, A., & Mizomov, M. (2021). Experimental study of the extraction process of coniferous plants. IOP Conference Series: Earth and Environmental Science, 839(4), 042019. https://doi.org/10.1088/1755-1315/839/4/042019
Dung, P. N. T., Dao, T. P., Le, T. T., Tran, H. T., Dinh, T. T. T., Pham, Q. L., Tran, Q. T., & Pham, M. Q. (2021). Extraction and analysis of chemical composition of Ocimum gratissimum L essential oil in the North of Vietnam. IOP Conference Series: Materials Science and Engineering, 1092(1), 012092. https://doi.org/10.1088/1757-899X/1092/1/012092
Ebadi, M. T., Sefidkon, F., Azizi, M., & Ahmadi, N. (2017). Packaging methods and storage duration affect essential oil content and composition of lemon verbena (Lippia citriodora Kunth.). Food Science and Nutrition, 5(3), 588–595. https://doi.org/10.1002/fsn3.434
Edo, G. I., Samuel, P. O., Ossai, S., Nwachukwu, S. C., Okolie, M. C., Oghenegueke, O., Asaah, E. U., Akpoghelie, P. O., Ugbune, U., Owheruo, J. O., Ezekiel, G. O., Onoharigho, F. O., & Agbo, J. J. (2023). Phytochemistry and pharmacological compounds present in scent leaf: A review. Food Chemistry Advances, 3, 100300. https://doi.org/10.1016/j.focha.2023.100300
Gulcin, İ., & Alwasel, S. H. (2023). DPPH Radical Scavenging Assay. Processes, 11(8), 2248. https://doi.org/10.3390/pr11082248
Guo, M., Qin, S., Wang, S., Sun, M., Yang, H., Wang, X., Fan, P., & Jin, Z. (2023). Herbal medicine nanocrystals: a potential novel therapeutic strategy. Molecules, 28(17), 6370. https://doi.org/10.3390/molecules28176370
Hamma, I. I., Tafinta, I. Y., Abdulmalik, A., Theophilus, J., & Abubakar, M. (2020). Phytochemical screening and antibacterial activity of the crude extract of scent leaf (Ocimum gratissimum) on Escherichia coli and Staphylococcus aureus. Asian Plant Research Journal, 1–7. https://doi.org/10.9734/aprj/2020/v5i230101
Ikpeazu, V. O., Emmanuel, O., Ekweogu, C. N., Akara, E. U., & Ugbogu, E. A. (2019). A comparative nutritional assessments of leaf extracts of Ocimum gratissimum and Solanum aethiopicum. American Journal of Biomedical Research, 7(2), 27–31. https://doi.org/10.12691/ajbr-7-2-1
Imosemi, I. O. (2020). A Review of the Medicinal Values , Pharmacological Actions , Morphological Effects and Toxicity of Ocimum Gratissimum Linn. European Journal of Pharmaceutical and Medical Research, 7(7), 29–40.
Jha, A. K., & Sit, N. (2022). Extraction of bioactive compounds from plant materials using combination of various novel methods: A review. Trends in Food Science & Technology, 119, 579–591. https://doi.org/10.1016/j.tifs.2021.11.019
Kavyamala, D., Jayamma, P., Rani, N. U., & Babu, M. M. (2023). Phytochemical screening of aqueous, acetone, ethanol and methanol leaf extracts of Ocimum teniflorum, Ocimum gratissimum and Ocimum sanctum. International Journal of Economic Plants, 10(May, 2), 109–113. https://doi.org/10.23910/2/2023.0515b
Klein-Junior, L. C., de Souza, M. R., Viaene, J., Bresolin, T. M. B., de Gasper, A. L., Henriques, A. T., & Heyden, Y. Vander. (2021). Quality control of herbal medicines: from traditional techniques to state-of-the-art approaches. Planta Medica, 87(12/13), 964–988. https://doi.org/10.1055/a-1529-8339
Kpètèhoto, H. W., Amoussa, A. M. O., , Roch Christian Johnson, E. E. M., Houéto, Mignanwandé, F. M. Z., Yédomonhan, H., Loko4, F., Bankolé, H., Latifou, & Lagnika. (2019). Phytochemical analysis and antioxidant potential of Ocimum gratissimum Linn (Lamiaceae) commonly consumed in the Republic of Benin. Journal of Applied Biology & Biotechnology, 7(4), 75–83. https://doi.org/10.7324/JABB.2019.70412
Kumar, L., Verma, S., Singh, M., Chalotra, T., & Utreja, P. (2018). Advanced drug delivery systems for transdermal delivery of non-steroidal anti-inflammatory drugs: a review. Current Drug Delivery, 15(8), 1087–1099. https://doi.org/10.2174/1567201815666180605114131
Lang, Y., Gao, N., Zang, Z., Meng, X., Lin, Y., Yang, S., Yang, Y., Jin, Z., & Li, B. (2024). Classification and antioxidant assays of polyphenols: a review. Journal of Future Foods, 4(3), 193–204. https://doi.org/10.1016/j.jfutfo.2023.07.002
Ojewumi, M. E., Babatunde, D. E., Orjiakor, T. G., Ojewumi, E. O., & Olawale-Success, O. O. (2024). Exploring the therapeutic potential of Ocimum gratissimum extracts against microbial infections: A Comprehensive review. Medicine India, 3, 45. https://doi.org/10.25259/MEDINDIA_11_2024
Okeke, L. A., Anie, C. O., Nwobodo, D. C., & Vitus Kasie Okolo. (2023). Phytochemical analysis and antibacterial activities of various leaf extracts of Ocimum gratissimum and Newbouldia laevis. GSC Biological and Pharmaceutical Sciences, 22(3), 144–152. https://doi.org/10.30574/gscbps.2023.22.3.0104
Olumide, M. D., Ajayi, O. A., & E. O. Akinboye. (2019). Comparative study of proximate, mineral and phytochemical analysis of the leaves of Ocimum gratissimum, Vernonia amygdalina and Moringa oleifera. Journal of Medicinal Plants Research, 13(15), 351–356. https://doi.org/10.5897/JMPR2019.6775
Pandiri, I., & Moni, A. (2018). Ocimum herb species: a potential treatment strategy for diabetic kidney disease. Journal of Advanced Biotechnology and Experimental Therapeutics, 1(3), 88. https://doi.org/10.5455/jabet.2018.d16
Pathak, A., Gupta, A. P., & Pandey, P. (2024). Herbal Medicine and Sustainable Development Challenges and Opportunities (pp. 1–26). https://doi.org/10.1007/978-3-031-21973-3_48-1
QCVN 8-2:2011/BYT. (2011). National technical regulation on the limits of heavy metals contamination in food.
Samadi, S., Saharkhiz, M. J., Azizi, M., Samiei, L., Karami, A., & Ghorbanpour, M. (2021). Single-wall carbon nano tubes (SWCNTs) penetrate Thymus daenensis Celak. plant cells and increase secondary metabolite accumulation in vitro. Industrial Crops and Products, 165(March), 113424. https://doi.org/10.1016/j.indcrop.2021.113424
Silva, L. de L., Heldwein, C. G., Reetz, L. G. B., Hörner, R., Moraes, D. P. de, Duarte, F. A., Flores, É. M. de M., Zanella, R., Pereira, A. M. S., & Heinzmann, B. M. (2015). Influence of extraction method on antibacterial activity of ethanolic extracts of Ocimum gratissimum L. Journal of Medicinal Plants Research, 9(7), 199–206. https://doi.org/10.5897/JMPR2014.5387
Sridevi, G., Ibrahim, M., Sembulingam, K., Srividya, S., & Prema, S. (2015). Evaluation of in vitro anti-inflammatory activity of Pergularia Daemia. World Journal of Pharmaceutical Research SJIF Impact Factor 5, 990(6), 1100–1108. www.wjpr.net
Taghizadeh, S. F., Karimi, G., Tzatzarakis, M., Tsakiris, I., Ahmadpourmir, H., Azizi, M., Afshari, A., Ghorani, V., Yarmohammadi, F., Tsatsakis, A., & Rezaee, R. (2022). Probabilistic risk assessment of exposure to multiple metals and pesticides through consumption of fruit juice samples collected from Iranian market. Food and Chemical Toxicology, 170(October), 113493. https://doi.org/10.1016/j.fct.2022.113493
Tuan Anh, T., Thi Duyen, L., My Hang, L., Duc Lam, T., Giang Bach, L., Chinh Nguyen, D., & Quoc Toan, T. (2019). Effect of drying temperature and storage time on Ocimum gratissimum Linn. leaf essential oil from Central Highlands, Vietnam. Materials Today: Proceedings, 18, 4648–4658. https://doi.org/10.1016/j.matpr.2019.07.449
Ugbogu, O. C., Emmanuel, O., Agi, G. O., Ibe, C., Ekweogu, C. N., Ude, V. C., Uche, M. E., Nnanna, R. O., & Ugbogu, E. A. (2021). A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.). Heliyon, 7(11), e08404. https://doi.org/10.1016/j.heliyon.2021.e08404
Vietnamese Pharmacopoeia. (2019). Vietnamese Pharmacopoeia (V). Medical publishing house.
Wang, S., Dong, G., & Sheng, C. (2019). Structural simplification of natural products. Chemical Reviews, 119(6), 4180–4220. https://doi.org/10.1021/acs.chemrev.8b00504
Wołosiak, R., Drużyńska, B., Derewiaka, D., Piecyk, M., Majewska, E., Ciecierska, M., Worobiej, E., & Pakosz, P. (2021). Verification of the conditions for determination of antioxidant activity by ABTS and DPPH Assays—a practical approach. Molecules, 27(1), 50. https://doi.org/10.3390/molecules27010050
Zahran, E. M., Abdelmohsen, U. R., Khalil, H. E., Desoukey, S. Y., Fouad, M. A., & Kamel, M. S. (2020). Diversity, phytochemical and medicinal potential of the genus Ocimum L. (Lamiaceae). Phytochemistry Reviews, 19(4), 907–953. https://doi.org/10.1007/s11101-020-09690-9
Zareiyan, F., & Khajehsharifi, H. (2022). In-vitro phytochemical analysis of essential oil and methanolic and hydromethanolic extracts of Ocimum gratissimum. Journal of Plant Biochemistry and Biotechnology, 31(4), 894–906. https://doi.org/10.1007/s13562-022-00768-3 | ||
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