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Optimization of MS liquid medium to increase saffron cell biomass | ||
| Journal of Horticulture and Postharvest Research | ||
| دوره 9، Issue 1 - شماره پیاپی 33، خرداد 2026، صفحه 45-58 اصل مقاله (1.4 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22077/jhpr.2025.8864.1473 | ||
| نویسندگان | ||
| Somaye Amini1، 2؛ Seyed Mahdi Ziaratnia* 3؛ Nasim Adibpour3، 4 | ||
| 1Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Badiee Saffron Production Agricultural Company, Mashhad, Iran | ||
| 3Department of Food Biotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran | ||
| 4Grownida Innovation Center, Mashhad, Iran | ||
| چکیده | ||
| Purpose: The objective of this study was to enhance the MS-based medium to maximize cell biomass obtained from saffron corm within a liquid culture system. Research Method: The initial experiment employed a factorial design to examine three key factors influencing cell biomass: total nitrogen concentrations (20, 40, and 60 mM), ammonium/nitrate ratios (100:0, 75:25, 50:50, 25:75, and 0:100), and sucrose concentrations (3, 6 and 9%). In the second experiment, we evaluated the effect of a basal liquid MS medium enriched with L-glutamine, L-cysteine, PVP, chitosan, and KH2PO4 on cell biomass. The optimized medium from Experiment 1 was further enhanced based on the results obtained from Experiment 2 in order to increase cell biomass. Its effects were then compared to those of the standard MS medium by measuring fresh weight of cells over a six-week period in Experiment 3. Findings: Results from the initial experiment demonstrated a significant increase in cell biomass (3.37 g) when using 3% sucrose with nitrate instead of ammonium. Lowering the nitrogen concentration from 60 mM to 40 mM significantly improved cell growth. Additionally, both PVP and KH2PO4 contributed to increased saffron cell fresh weight in the second experiment. However, it was noted that even at low concentrations, chitosan application significantly enhanced cell death. The findings from the third experiment revealed that the modified MS culture medium, combined with potassium phosphate, significantly enhanced cell biomass growth compared to the standard MS medium. Research Limitations: No limitations were identified during this study. Originality/Value: This study evaluated key factors affecting cell biomass in saffron, but future research should explore the production of saffron metabolites under these influences. | ||
| کلیدواژهها | ||
| Ammonium؛ Biomass؛ Nitrate؛ Sucrose؛ Total nitrogen | ||
| مراجع | ||
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Amini, S., Ziaratnia, S. M., & Hemmati, Kh. (2022). Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor. Plant Cell Tissue and Organ Culture, 149(10), 243-255. 10.1007/s11240-022-02233-4
Amini, S., Ziaratnia, S. M., & Rajabzadeh, Gh. (2023). Improvement of friable callus induction of Crocus sativus L. and establishment of a cell suspension culture system with high biomass. Journal of Horticulture and Postharvest Research, 6(4), 397-408. https://doi.org/10.22077/jhpr.2023.6850.1338
Amirkavei Najafabadi, B., Qavami, N., Ebrahimi, M. A., Ebrahimi, P., & Zarinpanjeh, N. (2020). Enhancement of taxol production by applying amino acid complex along with chitosan in suspension culture of Taxus baccata L. Journal of Medicinal Plants, 19(76), 99-109. https://doi.org/10.29252/jmp.19.76.99
Bapat, V. A., Kavi Kishor, B. P., Jalaja, N., Jain, Sh. M., & Penna, S. (2023) Plant cell cultures: biofactories for the production of bioactive compounds. Agronomy, 13(3), 858. https://doi.org/10.3390/agronomy13030858
Britto, D. T., & Kronzucker, H. J., (2002). NH4+ toxicity in higher plants: a critical review. Journal of Plant Physiology, 159(6), 567-584. https://doi.org/10.1078/0176-1617-0774
Cai, X., Wei, H., Liu, Ch., Ren, X., Thi, L., & Jeon, B. R., (2020). Synergistic effect of NaCl pretreatment and PVP on browning suppression and callus induction from petal explants of Paeonia lactiflora Pall. ‘Festival maxima’. 9(3),346. https://doi.org/10.3390/plants9030346
Chang, J., Liu, D., Cao, H., Chang, S. X., Wang, X., Huang, C., & Ge, Y. (2010). NO3−/NH4+ ratios affect the growth and N removal ability of Acorus calamus and Iris pseudacorus in a hydroponic system. Aquatic Botany, 93(4), 216-220. https://doi.org/10.1016/j.aquabot.2010.08.002
Chen, S., Wang, X., Zhao, B., Yuan, X., & Wang, Y. (2003). Production of crocin using Crocus sativus callus by two-stage culture system. Biotechnology Letters, 25(15), 1235-1238. https://doi.org/10.1023/A:1025036729160
Chewapanich, W., Charoenrak, P., Intanoo, W., & Chamswarng, C. (2021). Efficacy of Trichoderma asperellum CB-Pin-01 and potassium dihydrogen phosphate to enhance growth and yield and reduce Pythium root rot of hydroponically grown lettuce. Agriculture and Natural Resources, 55, 601–610. https://doi.org/10.34044/j.anres.2021.55.4.10
Cui, X. H., Murthy, H. N., Wu, C. H., & Paek, K. Y. (2010). Sucrose-induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell Tissue Organ Culture, 103(1), 7-14. https://doi.org/10.1007/s11240-010-9747-z
Farjaminezhad, R., & Garoosi, Gh. (2021). Prediction of the effect of chitosan on cell suspension culture of Azadirachta indica by response surface methodology. Plant Cell Tissue and Organ Culture, 146, 323-337. https://doi.org/10.1007/s11240-021-02072-9
Fazal, H., Abbasi, B. H., Ahmad, N., Ali, M., & Ali, S. (2016). Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L. Plant Cell Tissue Organ Culture, 124(3), 573-581. https://doi.org/10.1007/s11240-015-0915-z
Garnica, M., Houdusse, F., Yvin, J. C., & Garcia‐Mina, J. M. (2009). Nitrate modifies urea root uptake and assimilation in wheat seedlings. Journal of the Science of Food and Agriculture, 89(1), 55-62. https://doi.org/10.1002/jsfa.3410
George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant Propagation by Tissue Culture: The components of plant tissue culture media I: macro-and micro-nutrients. Chapter 3. 65-113. https://doi.org/10.1007/978-1-4020-5005-3_3
Ghosh, S., Ghosh, B., & Jha, S. (2016). Role of exogenous carbohydrate and amino acid sources on biomass and colchicine production in nontransformed root cultures of Gloriosa superba. Plant Tissue Culture and Biotechnology, 25(2), 247-256. http://dx.doi.org/10.3329/ptcb.v25i2.26258
Golmohammadi, F. (2014). Saffron and its farming, economic importance, export, medicinal characteristics and various uses in South Khorasan Province-East of Iran. International Journal of Food and Allied Sciences, 3(5), 566-596.
Gopi, C., & Vatsala, T. (2006). In vitro studies on effects of plant growth regulators on callus and suspension culture biomass yield from Gymnema sylvestre R. Br. African Journal of. Biotechnology, 5(12), 1215-1219. http://www.academicjournals.org/AJB
Gresta, F., Lombardo, G., Siracusa, L., & Ruberto, G. (2009). Saffron, an alternative crop for sustainable agricultural systems: a review. Agronomy for Sustainable Development, 28(1), 355-376. https://doi.org/10.1007/978-90-481-2666-8_23
Hachiya, T., & Sakakibara, H. (2016). Interactions between nitrate and ammonium in their uptake, allocation, assimilation, and signaling in plants. Journal of Experimental Botany, 68(10), 2501-2512. https://doi.org/10.1093/jxb/erw449
Hachiya, T., Watanabe, C. K., Fujimoto, M., Ishikawa, T., Takahara, K., Kawai-Yamada, M., Uchimiya, H., Uesono, Y., Terashima, I., & Noguchi, K. (2012). Nitrate addition alleviates ammonium toxicity without lessening ammonium accumulation, organic acid depletion and inorganic cation depletion in Arabidopsis thaliana shoots. Plant and Cell Physiology, 53(3), 577-591. https://doi.org/10.1093/pcp/pcs012
Hawkesford, M., Horst, W., Kichey, T., Lambers, H., Schjoerring, J., Moller, I.S., & White, PH. (2012). Marschner's mineral nutrition of higher plants. Chapter 6 - Functions of Macronutrients. 135-189. https://doi.org/10.1016/B978-0-12-384905-2.00006-6
Heydari, H. R., Chamani, E., & Esmaeilpour, B. (2020). Effect of total nitrogen content and NH4+/NO3- ratio on biomass accumulation and secondary metabolite production in cell suspension culture of Salvia nemorosa. Iranian Journal of Genetics and Plant Breeding, 9(1), 17-27. https://doi.org/10.30479/ijgpb.2020.12321.1258
Husaini, A. M., Kamili, A. N., Wani, M., Teixeira, d. S. J., & Bhat, G. (2010). Sustainable saffron (Crocus sativus Kashmirianus) production: technological and policy interventions for Kashmir. Functional Plant Science and Biotechnology, 4(2), 116-127.
Keng, C. L., Wei, A. S., & Bhatt, A. (2010). Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia. UNED Research Journal, 2(2), 239-244. http://dx.doi.org/10.22458/urj.v2i2.160
Lachguer, Kh., Boudadi, I., Lachheb, M., Beraouz, I., Merzougui, S. El., Caid, M. B. El., Lagram, Kh., Serghini, M. A. (2025). Saffron (Crocus sativus L.) Cultivation and Properties: A Review. International Journal of Horticultural Science and Technology,12(2), 627-646. https://doi.org/10.22059/ijhst.2024.377028.855
Liu, S., & Zhong, J. J. (1996). Effects of potassium ion on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax ginseng. Journal of Biotechnology, 52(2), 121-126. https://doi.org/10.1016/S0168-1656(96)01632-X
Lu, Y. L., Xu, Y.C., Shen, Q. R., & Dong, C. X. (2009). Effects of different nitrogen forms on the growth and cytokinin content in xylem sap of tomato (Lycopersicon esculentum Mill.) seedlings. Plant Soil, 315(1-2), 67-77. https://doi.org/10.1007/s11104-008-9733-y
Matsubayashi, Y., & Sakagami, Y. (1998). Effects of the medium ammonium-nitrate ratio on competence for asparagus cell division induced by phytosulfokine-α. Plant Cell Reports, 17(5), 368-372. https://doi.org/10.1007/s002990050408
Maximo, W. P. F., Almeida Santos, P. A., Mendonça, E. G., Santos, B. R., & Paiva, L. V. (2015). Nitrate (NO3-) and ammonium (NH4+) ratios for propagation of 'Eucalyptus' hybrid in two different 'in vitro' cultivation systems. Australian Journal of Crop Science, 9(12), 1242-1248.
Mofid Bojnoordi, M. (2022). The effect of different concentrations of chitosan on the production of phenolic acids in cell culture of lactuca undulate ledeb. Agricultural Biotechnology Journal, 14 (3),1-20. DOI: 10.22103/jab.2022.18773.1369.
Ranandi, A., Golizadegan ehsanabad, A. & Seifi, A. (2022). Optimization of callogenesis and cell suspension culture in saffron. Saffron Research Journal, 10(2), 276-284. https://doi.org/10.22077/JSR.2022.5718.1198.
Shah, M., Jan, H., Drouet, S., Tungmunnithum, D., Shirazi, J. H., Hano, Ch., & Abbasi, B. H. (2021). Chitosan elicitation impacts flavonolignan biosynthesis in Silybum marianum (L.) gaertn cell suspension and enhances antioxidant and anti-Inflammatory activities of cell extracts. Molecules, 26(4), 791-808. https://doi.org/10.3390/molecules26040791.
Sharma, K. D., & Piqueras, A. (2010). Saffron (Crocus sativus L.) tissue culture: micropropagation and secondary metabolite production. Functional Plant Science and Biotechnology, 4(2), 15-24.
Shi, L., Liang, J., Wang, R., Wan, X., Yan, B., Zhang, Y., Chen, M., Liu, Ch., Li, Q., Wang, Sh., & Guo, L. (2023). The ammonium/nitrate ratio affects the growth and shikonin accumulation in Arnebia euchroma. Agronomy, 13 (5), 1-18 . https://doi.org/10.3390/agronomy13051318
Sivanandhan, G., Dev, G. K., Jeyaraj, M., Rajesh, M., Muthuselvam, M., Selvaraj, N., Manickavasagam, M., & Ganapathi, A. (2013). A promising approach on biomass accumulation and withanolides production in cell suspension culture of Withania somnifera (L.) Dunal. Protoplasma, 250(4), 885-898. https://doi.org/10.1007/s00709-012-0471-x
Suan See, K., Bhatt, A., Lai Keng, C. (2011). Effect of sucrose and methyl jasmonate on biomass and anthocyanin production in cell suspension culture of Melastoma malabathricum (Melastomaceae). Revista de Biologia Tropical, 59 (2), 597-606.
Supatmi, S., & Endang, A. (2007). The effects of low phosphorus concentration in MS medium on callus growth and reserpine production of pule pandak Rauvolfia verticillata in vitro. Biofarmasi, 5(1), 16-25. https://doi.org/10.13057/biofar/f050103
Veliky, I., & Rose, D. (1973). Nitrate and ammonium as nitrogen nutrients for plant cell cultures. Canadian Journal of Botany, 51(10), 1837-1844. https://doi.org/10.1139/b73-235
Xu, J., Ge, X., & Dolan, M. C. (2011). Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. Biotechnology Advances, 29(3), 278-299. https://doi.org/10.1016/j.biotechadv.2011.01.002
Zhu, Z., Y, M., Chen, Y., Guo, Q. Zhang, L., Shi, H., & Liu, L. (2014). Effects of ammonium to nitrate ratio on growth, nitrogen metabolism, photosynthetic efficiency and bioactive phytochemical production of Prunella vulgaris. Pharmaceutical Biology, 52(12), 1518-1525. https://doi.org/10.3109/13880209.2014.902081
Ziaratnia, S. M., & Amini, S. (2021). The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L. Journal of Horticulture and Postharvest Research, 4(special issue: recent advances in saffron), 43-56. https://doi.org/10.22077/jhpr.2021.3684.1166 | ||
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