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Improvement of friable callus induction of Crocus sativus L. and establishment of a cell suspension culture system with high biomass | ||
| Journal of Horticulture and Postharvest Research | ||
| دوره 6، Issue 4 - شماره پیاپی 23، اسفند 2023، صفحه 397-408 اصل مقاله (1.19 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22077/jhpr.2023.6850.1338 | ||
| نویسندگان | ||
| Somaye Amini1؛ Seyed Mahdi Ziaratnia* 2؛ Ghadir Rajabzadeh3 | ||
| 1Department of Horticultural Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Department of Food Biotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran | ||
| 3Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran | ||
| چکیده | ||
| Purpose: This study aims to explore the potential of in vitro culture as a method for scaling up the production of saffron based medicinal compounds, the most expensive spice renowned. Emphasis is placed on the critical role of friable callus (FC) formation as a prerequisite for successful suspension culture. Research method: The research primarily investigates FC formation, focusing on the impact of varying strengths of Murashige and Skoog (MS) medium as well as combinations of NAA or 2,4-D and BA or Kin on compact callus. Subsequently, the study involves supplementing the MS medium with different concentrations of 2,4-D, kin, zeatin, glutamine, sucrose, and nitrogen to establish a cell suspension culture. Findings: The highest FC yield was achieved on a solid medium containing 2,4-D (1 mg l-1)+Kin (0.2 mg l-1), resulting in a fresh weight (FW) of 0.413 g. Furthermore, MS combined with 2,4-D (1 mg l-1)+Kin (0.2 mg l-1)+glutamine (10 mg l-1), as well as MS+2,4-D (0.5 mg l-1)+zeatin (0.3 mg l-1)+glutamine (10 mg l-1), demonstrated the highest FW under suspension conditions. The study also identified that 30 g l-1 sucrose and 30 µM were optimal for inducing maximum FW. Research limitations: Cell biomass is influenced by several factors that should to be optimized. Originality/Value: This research concludes that a cell suspension system holds promise for rapidly generating sufficient cell biomass to produce valuable secondary metabolites within a limited timeframe and space. Notably, the system successfully increased biomass from 0.2 to 1.2 g, underscoring its potential for efficient saffron-based product development. | ||
| کلیدواژهها | ||
| Callogenesis؛ Glutamine؛ MS medium؛ Saffron؛ Solid medium | ||
| مراجع | ||
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