
تعداد نشریات | 21 |
تعداد شمارهها | 301 |
تعداد مقالات | 3,173 |
تعداد مشاهده مقاله | 3,211,773 |
تعداد دریافت فایل اصل مقاله | 2,380,294 |
Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion | ||
Journal of Horticulture and Postharvest Research | ||
مقاله 3، دوره 4، Issue 3 - شماره پیاپی 14، آذر 2021، صفحه 277-288 اصل مقاله (1.57 M) | ||
نوع مقاله: Original Article | ||
شناسه دیجیتال (DOI): 10.22077/jhpr.2020.3665.1163 | ||
نویسندگان | ||
Rahele Dehghani1؛ Zahra Abbasi* 2؛ Majid Talebi1؛ Emad Shahmansouri2؛ Badrodin Ebrahim Sayed Tabatabaei1 | ||
1Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, 8415683111 | ||
2Horticulture Crops Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, (AREEO), Isfahan, Iran | ||
چکیده | ||
Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in 123 onion accessions. Research method: Three cytoplasmic markers cob, accD and MK were used to identify the sterility (S) from the fertility (N) cytoplasm and four nuclear molecular markers (OPT, PsaO, Jnurf-13 and AcSKP1) were used for genotyping of Ms alleles. Findings: The results showed that the two accD and cob markers were quite similar in the detection of the type of cytoplasm with 100% male sterility for male sterile lines and 100% fertility for maintainer lines. The MK marker was able to distinguish T-type cytoplasm as well. Based on the results, the frequency of fertility (N) was much more than the frequency of sterility (S and T) cytoplasm found to be 90% in Dorche (pop.1), 100% in Dorche (pop.2) and Kashan based on marker cob and accD and with MK marker, was found to be 80%, 90% and 82% in Dorche (pop.1), Dorche (pop.2) and Kashan, respectively. Limitations: In this study, nuclear markers were not successful due to not finding linkage disequilibrium with the Ms locus, suggested more markers to be evaluated. Originality/Value: Molecuar markers were very suitable for the indentification of S or N lines. Cytotype (N/S) determination of plants by usingmolecular markers (cob, accD and MK), could easily reduce the population size required for the production of onion hybrid seeds. | ||
کلیدواژهها | ||
Allium cepa؛ Cytoplasmic male sterility؛ Hybrid؛ Restoration locus | ||
مراجع | ||
Bang, H., Cho, D. Y., Yoo, K., Yoon, M., Patil, B. S. & Kim, S. (2011). Development of simple PCR-based markers linked to the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.). Euphytica, 179, 439-449. https://doi.org/10.1007/s10681-010-0342-5. Cho, K. S., Yang, T., Hong, S., Kwon, Y., Woo, J. & Park, H. (2006). Determination of cytoplasmic male sterile factors in onion plants (Allium cepa L.) using PCR-RFLP and SNP markers. Molecular Cells, 21, 411-417. Engelke, T., Terefe, D. & Tatlioglu, T. (2003). A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theoretical and Applied Genetics, 107, 162-167. https://doi.org/10.1007/s00122-003-1230-3. Ferreira, R. R. & Santos, C. A. F. (2018). Partial success of marker-assisted selection of ‘A’and ‘B’onion lines in Brazilian germplasm. Science Horticulture, 242, 110-115. https://doi.org/10.1016/j.scienta.2018.08.002. Ferreira, R. R., Santos, C. A. F. & Oliveira, V. R. (2017). Fertility restoration locus and cytoplasm types in onion. Genetics and Molecular Research, 16(3),1-12. Gökçe, A. F. & Havey, M. J. (2002) Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations. Journal of the American Society for Horticultural Science, 127, 944-946. https://doi.org/10.21273/jashs.127.6.944. Havey, M. J. (1993). A putative donor of S-cytoplasm and its distribution among open-pollinated populations of onion. Theoretical and Applied Genetics, 86, 128-134. https://doi.org/10.1007/bf00223817. Havey, M. J. & Bark O. H. (1994). Molecular confirmation that sterile cytoplasm has been introduced into open-pollinated Grano onion cultivars. Journal of the American Society for Horticultural Science. 119, 90-93. https://doi.org/10.21273/jashs.119.1.90. Havey, M. J. (2000). Diversity among male-sterility-inducing and male-fertile cytoplasms of onion. Theoretical and Applied Genetics, 101,778-782. https://doi.org/10.1007/s001220051543. Havey, M. J. (1995). Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open- pollinated populations of onion. Theoretical and Applied Genetics, 90, 263-268. https://doi.org/10.1007/bf00222212. Havey, M. J. (2013). Single nucleotide polymorphisms in linkage disequilibrium with the male-fertility restoration (ms) locus in open-pollinated and inbred populations of onion. Journal of the American Society for Horticultural Science, 138, 306-309. https://doi.org/10.21273/jashs.138.4.306. Huo, Y. M., Liu, B. J., Yang, Y., Miao, J., Gao, L. M., Kong, S. P., Wang, Z. B., Kitano, H. & Wu, X. (2015). AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion. Eurhytica, 204, 711-722. https://doi.org/10.1007/s10681-015-1374-7. Jones, H. A. & Clarke, A. E. (1943). Inheritance of male sterility in the onion and the production of hybrid seed. Proc. Journal of the American Society for Horticultural Science 43, 189–194. Jones, H. A., & Davis, G. (1944). Inbreeding and heterosis and their relation to the development of new varieties of onions. USDA Tech Bull 874, 1-28. Jones, H. A., & Emsweller, S. L. (1936). A male-sterile onion. Proceedings of the American Society for Horticultural Science, 34, 582–585. Khar, A. & Saini, N. (2016). Limitations of PCR-based molecular markers to identify male-sterile and maintainer plants from Indian onion (Allium cepa L.) populations. Plant Breeding, 135, 519-524. https://doi.org/10.1111/pbr.12373. Khosa, J. S., McCallum, J., Dhatt, A. S., & Macknight, R. C. (2016). Enhancing onion breeding using molecular tools. Plant Breeding, 135, 9-20. https://doi.org/10.1111/pbr.12330 Kim, S. (2014) A codominant molecular marker in linkage disequilibrium with a restorer-of fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Molecular Breeding, 34, 769-778. https://doi.org/10.1007/s11032-014-0073-8. Kim, S., Kim, C. W., Park, M. & Choi, D. (2015a). Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq. Theoretical and Applied Genetics, 128, 2289-2299. https://doi.org/10.1007/s00122-015-2584-z. Kim, S., Lee, E. T., Cho, D. Y., Han, T., Bang, H., Patil, B. S., Ahn, Y. K., & Yoon, M. K. (2009). Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.). Theoretical and Applied Genetics, 118, 433–441. https://doi.org/10.1007/s00122-008-0909-x. Kim, S., Park, J., & Yang, T. (2015b). Comparative analysis of the complete chloroplast genome sequences of a normal male-fertile cytoplasm and two different cytoplasms conferring cytoplasmic male sterility in onion (Allium cepa L.). Journal Horticulture Science and Biotechnology, 90, 459-468. https://doi.org/10.1080/14620316.2015.11513210. Malik, G., Dhatt, A. S. & Malik, A. A. (2017). Isolation of Male Sterile and Maintainer Lines from North-Indian Onion (Allium cepa L.) Populations with the Aid of PCR-Based Molecular Marker. Vegetos-An International Journal of Plant Research, 30(2), 94-99. https://doi.org/10.5958/2229-4473.2017.00142.2. Murry, M., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research, 8, 4321-4325. Patil, Y. A., Dalvi, V. S., Borole, V. K., Krishna, B., Dhake, A. V., Sane, P. V. & Sane, A. P. (2016). Molecular characterization of CMS lines in short-day white onions. Acta Horticulture, 1143, 259-268. https://doi.org/10.17660/actahortic.2016.1143.37. Pike, L. M. (1986). Onion breeding. In: Basset, M. J. (Ed.), Breeding Vegetable Crops. AVI Publishing Company, Westport pp: 357-394. Ragassi, C. F., Santos, M. D. M., Fonseca, M. E. N., Oliveira, V. R., Buzar, A. G. R., Costa, C. P. & Boiteux, L. S. (2012). Genotyping of polymorphisms associated with male-sterility systems in onion accessions adapted for cultivation in Brazil. Horticultura Brasileira, 30, 409-414. https://doi.org/10.1590/s0102-05362012000300009. Santos, C. A. F., Leite, D. L., Oliveira, V. R. & Rodrigues, M. A. (2010). Marker-assisted selection of maintainer lines within an onion tropical population. Scientia Agricola, 67, 223-227. https://doi.org/10.1590/s0103-90162010000200015. Santos, M. D. M., Buzar, A. G. R., Fonseca, M. E. N., Oliveira, V. R., Torres, A. C. & Boiteux, L. S. (2007). Molecular characterization of cytoplasm type (N, S, and T) in accessions of an onion germplasm collection from Brazil. Horticultura Brasileira, 25(1), 1-4. https://doi.org/10.1590/s0102-05362007000400007 Sato, Y. (1998). PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.). Theoretical and Applied Genetics, 96, 367-370. https://doi.org/10.1007/s001220050750. Schweisguth, B. (1973). Study of a new type of male sterility in onion (Allium cepa L.). Ann Amelior Plant. 23, 221-253. Shahmansouri, E. & Aminpur, R. (2004). Investigation on the possibility of recognition and non-sexual reproduction of male sterile genotypes in three landrace populations. Final report. Isfahan Agricultural and Natural Resources Research and Education Center, AREEO 16 page. Shahmansouri, E. & Otroshi, M. (2011). Micropropagation of onion male sterile genotypes. Final report. Institute of Agricultural Biotechnology 34 page. Terefe, D. T. E. & Tatlioglu, T. (2003). A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L). Theoretical and Applied Genetics, 107, 162-167. https://doi.org/10.1007/s00122-003-1230-3. Tsujimura, M., Kaneko, T., Sakamoto, T., Kimura, S., Shigyo, M., Yamagishi, H. & Terachi, T. (2019). Multichromosomal structure of the onion mitochondrial genome and a transcript analysis. Mitochondrion, 46, 179-186. https://doi.org/10.1016/j.mito.2018.05.001. Von Kohn, C., Kiełkowska, A. & Havey, M. J. (2013). Sequencing and annotation of the chloroplast DNAs and identification of polymorphisms distinguishing normal male-fertile and male-sterile cytoplasms of onion. Genome, 56 (12), 737-742. https://doi.org/10.1139/gen-2013-0182. | ||
آمار تعداد مشاهده مقاله: 775 تعداد دریافت فایل اصل مقاله: 587 |